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Recombinant Antibody Library

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Recombinant Antibody Library

Rx Biosciences offers construction of mouse, human and rabbit scFv and Fab libraries in a phagemid vector. Either vL or vH are cloned separately or vL and vH are cloned together, separated by a linker region.  The libraries are good for screening and production of monoclonal antibodies.

 

Features:

  • scFv Light Chain (vL) antibody library in a phagemid vector.
  • scFv Heavy Chain (vH) antibody library in a phagemid vector.
  • Construction of recombinant vL and vH chimeric antibodies in a phagemid vector.
  • Construction of Fab antibody library.
  • Construction of (Fab) 2 antibody library.
  • Construction of a library of nanobodies.
  • Construction of a bi-specific antibody library (with both domains, recognizing different targets).
  • Construction of a bi-specific ligand-antibody conjugate (one domain acts like an antibody while other domain acts like a ligand).
  • Construction of camelid and IgNAR antibody libraries.
Expression strategies:

  • Secreted expression in periplasm of E. coli.
  • Soluble expression in cytoplasm of E. coli.
  • Insoluble expressed as inclusion bodies.
  • Secreted expression in 293 and CHO cells.
Purification strategies:

  • Affinity tag: 6xHis tag.
  • Molecular format: insertion of SUMO between His6 tag and protein of interest (POI).
  • Purification: IMAC (Immobilized Metal Affinity Chromatography).
  • Tag removal after purification: cleavage at the C terminus of SUMO with SUMO protease.

To receive a custom quote, please contact us. We look forward to hearing from you!

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