Normalized cDNA Library
Rx Biosciences constructs normalized cDNA libraries in which high-copy gene sequences are selectively removed resulting into the enrichment of low copy and rare genes in the library. As per customer’s request we employ either the technology in which the double stranded cDNA clones of a regular library are converted into single stranded circles, hybridized with cRNA and unhybridized singled stranded circular plasmids are converted into double stranded plasmids or the normalization of high copy genes is performed at the mRNA level before library construction. PCR based technique is also used as per customer’s request and sample requirement.
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