Our Phage Display Technology

Rx Biosciences provides comprehensive phage display services for antibody discovery, protein engineering, and therapeutic development. Our platform combines advanced library construction with high-throughput screening to identify high-affinity binders against virtually any target.

Using M13 and T7 phage display systems, we generate highly diverse peptide, antibody and cDNA libraries and employ iterative panning, sequencing, and functional characterization to rapidly isolate lead antibody candidates.

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Ready-to-Go Display Libraries

Accelerate your antibody and DARPin discovery with Rx Biosciences' ready-to-screen, high-diversity display libraries. Our validated libraries eliminate the time and cost of library construction, enabling rapid identification of high-affinity binders for research, diagnostics, and therapeutic development.

Available Libraries

Human Naïve scFv Phage Display Library

(Complexity >1 × 10¹²)

Chicken Naïve scFv Phage Display Library

(Complexity >1 × 10¹⁰)

Ready-to-Go Display Libraries

Accelerate your antibody and DARPin discovery with Rx Biosciences' ready-to-screen, high-diversity display libraries. Our validated libraries eliminate the time and cost of library construction, enabling rapid identification of high-affinity binders for research, diagnostics, and therapeutic development.

Available Libraries

Human Naïve scFv Phage Display Library

(Complexity >1 × 10¹²)

Chicken Naïve scFv Phage Display Library

(Complexity >1 × 10¹⁰)

High-Diversity Libraries

Libraries with diversity of 1010 - 1012 unique clones.

Target-Specific Biopanning

Iterative panning to enrich for the strongest binders.

ELISA - SPR - FACS Validation

Multiple orthogonal methods to validate binding affinity.

scFv - Fab - VHH Libraries

Comprehensive antibody formats for diverse applications.

Fast Lead Discovery

3-6 weeks turnaround from library to hit identification.

OUR WORKFLOW

Library Construction

Construction of high-diversity library of small peptides, cDNAs, antigens, scFvs and Fabs.

Target Biopanning

Target-specific biopanning to enrich high-affinity binder.

Clone Selection & DNA Sequencing

Clone isolation and DNA sequencing.

Antibody Expression & Purification

Expression and purification of scFv and Fab antibodies.

Affinity Validation

Binding affinity validation using ELISA, FACS, SPR (Biacore), and flow cytometry.

Lead Optimization

Integration with yeast display for affinity maturation and improved antibody performance.

To further enhance lead optimization, we can seamlessly integrate phage display with
yeast display for affinity maturation and improved antibody performance.

SERVICE HIGHLIGHTS
  • Construction of cDNA, peptide, scFv, Fab, and VHH (nanobody) phage display libraries
  • Library diversity ranging from 10¹⁰–10¹² unique clones
  • Human, mouse, rabbit, chicken, and llama antibody libraries
  • Custom peptide libraries for PhIP (Phage ImmunoPrecipitation) applications
  • Screening of NEB Ph.D.™-7, Ph.D.™-12, and Ph.D.™ C7C peptide libraries
  • High-affinity binder identification through iterative biopanning
  • Expression and purification of scFv and Fab antibodies
  • Conversion of antibody fragments into human IgG for mammalian expression
  • Binding validation using ELISA, Biacore (SPR), and Flow Cytometry
APPLICATIONS
  • Therapeutic Antibody Discovery
  • Monoclonal Antibody Development
  • Nanobody (VHH) Discovery
  • Peptide Library Screening
  • Protein Engineering
  • Epitope Mapping
  • Affinity Maturation
  • Biomarker Discovery
PLATFORM ADVANTAGES
  • High-diversity libraries (10¹⁰–10¹²)
  • Illumina MiSeq NGS platform
  • Validation by flowcytometry, ELISA and Biacore.
  • Integrated ribosome display, phage and yeast display workflows (combinatorial screening)
  • Comprehensive downstream characterization and validation
  • Experienced scientific team with decades of antibody, ligands, targets, receptors discovery expertise
  • Experienced scientific support from project design through lead selection

Accelerate Your Antibody Discovery

Partner with Rx Biosciences for custom phage display solutions, from library construction through lead validation and antibody optimization.