Rx Biosciences provides comprehensive custom cDNA library construction services for researchers studying gene expression, transcript diversity, functional genomics, and novel gene discovery.
We construct high-quality cDNA libraries from a wide range of biological sources, including tissues, cultured cells, blood, plants, microorganisms, and rare or difficult-to-obtain samples. Our optimized workflows are capable of working with micro-quantities of total RNA, making them well suited for precious samples such as patient specimens, laser-capture microdissected tissues, and sorted cell populations.
Depending on your research goals, libraries can be prepared as full-length, normalized, subtracted, or conventional cDNA/EST libraries. Each project is customized around your starting material, desired transcript representation, downstream application, and preferred cloning vector.
Every cDNA library project is designed around the biological sample and the intended downstream application. Our scientists can optimize RNA preparation, cDNA synthesis, size selection, cloning strategy, library amplification, and quality-control procedures to maximize library complexity while minimizing representation bias.
Libraries can be constructed using customer-selected vectors, including specialized expression, screening, and functional-assay systems.
Our capabilities also allow us to work with challenging samples where starting RNA is limited, degraded, unusually GC-rich, or derived from rare biological material.
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.