Yeast Display
Rx Biosciences provides expert yeast display screening services, including advanced library construction and antibody selection. Yeast display (YD), also known as yeast surface display (YDP), is a highly effective technique for presenting proteins on the yeast cell surface after translation and proper maturation in a eukaryotic system. This screening method allows researchers to study antibody-antigen interactions using structurally relevant proteins.
Unlike phage display, yeast display operates in a eukaryotic environment, which supports proper protein folding, post-translational modification, and surface translocation. Consequently, yeast-displayed antibodies resemble their native human counterparts more closely. By using flow cytometry (FACS), scientists can precisely screen large libraries of antibodies displayed on yeast, significantly improving the discovery process.
For over 20 years, Rx Biosciences has developed and refined its proprietary yeast transformation protocol. This method yields up to 2×10⁸ transformants per microgram of ligation mixture, which facilitates the generation of high-diversity libraries. These robust libraries play a vital role in high-affinity antibody discovery and affinity maturation.
Our Approach
At Rx Biosciences, we actively monitor antibody maturation through yeast display screening. The yeast-expressed antibodies undergo binding with their target antigen under gradually increasing stringency. Using Fluorescence-Assisted Cell Sorting (FACS), we then isolate yeast cells that bind strongly to the antigen. Afterward, selected antibodies are purified via affinity chromatography for further study.
Moreover, our team works closely with researchers to tailor each screening project to specific discovery goals. This hands-on approach, combined with our unique protocols, ensures efficient and reproducible results.
Rx Biosciences’ yeast display screening platform is an ideal solution for those aiming to discover and refine high-affinity antibodies through a powerful, high-throughput system.
Service Highlights
- Therapeutic antibody discovery
- Monoclonal antibody discovery
- Antibody Humanization
- Antibody Affinity Maturation
- Protein-protein interaction
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