Phage Display Surface Display
Rx Biosciences provides quality custom phage display library construction and screening services for biological research and drug discovery related projects. The gene of interest is randomly mutated to produce various combinations of the peptides or small antibodies [e.g. scFv and Fab] or proteins which get displayed on the surface of a filamentous phage [M13, fd, and f1 strains] as fusion proteins. Using a binding affinity-based process called panning; a small number of phages that display proteins specifically binding to a target of interest are recovered from the phage library that usually has a repertoire of many billions of unique displayed proteins. Finally, the proteins displayed by the selected phages are identified by phage amplification followed by DNA sequencing.
Our Approach
Rx Biosciences uses yeast display system for the monitoring of the antibody maturation. An antibody expressed at the surface is allowed to bind to the antigen at increasing stringency and antigen-binding yeast cell population is sorted out using Fluorescence Assisted Cell Sorting (FACS). The antibody is then purified over an affinity column.
Service Highlights
- Construction of phage display cDNA library
- Construction of phage display peptide library with 1×10^8 to 1x10^12 diversity
- Panning of the library clones by affinity chromatography and amplification (4 rounds)
- Screening of Ph.D. 7, Ph.D. 12 and Ph.D. C7C libraries from NEB
- Screening of phage display cDNA library
- Expression and purification of ScFV and Fab antibodies in bacterial cells
- Conversion of ScFv & Fab antibodies into humanized IgGs and expression in mammalian cells
Request a Quote
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