Phage Display Surface Display
Rx Biosciences provides quality custom phage display services, including library construction and screening for biological research and drug discovery projects. We randomly mutate the gene of interest to generate various combinations of peptides, small antibodies (e.g., scFv and Fab), or proteins. These proteins are displayed on the surface of filamentous phages (M13, fd, and f1 strains) as fusion proteins.
Through a binding affinity-based process called panning, we recover a small number of phages that display proteins specifically binding to a target of interest. Typically, this selection occurs from a phage library that contains billions of unique displayed proteins. After selection, we identify the proteins displayed by the selected phages using phage amplification, followed by DNA sequencing.
Our Approach
At Rx Biosciences, we also use the yeast display system to monitor antibody maturation. We express the antibody on the cell surface and allow it to bind to the antigen under increasing stringency. Then, we sort the antigen-binding yeast cell population using Fluorescence-Assisted Cell Sorting (FACS). Afterward, we purify the selected antibody over an affinity column.
Our phage display services complement this yeast display approach by enabling the identification of high-affinity binding proteins efficiently.
Service Highlights
- Construction of phage display cDNA library
- Construction of phage display peptide library with 1×10^8 to 1x10^12 diversity
- Panning of the library clones by affinity chromatography and amplification (4 rounds)
- Screening of Ph.D. 7, Ph.D. 12 and Ph.D. C7C libraries from NEB
- Screening of phage display cDNA library
- Expression and purification of ScFV and Fab antibodies in bacterial cells
- Conversion of ScFv & Fab antibodies into humanized IgGs and expression in mammalian cells
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