Bacterial Display
Rx Biosciences constructs and screens custom bacterial display libraries of small peptides for various applications. These libraries support ligand discovery, antibody-antigen binding studies, and target identification. We use flow cytometry and standard selection procedures like biopanning to screen polypeptides displayed on the bacterial surface.
To create the library, we combine a highly diverse set of synthetically randomized peptide sequences using our proprietary technique. This process eliminates unwanted stop codons and sequences prone to aggregation. Therefore, the resulting library offers both diversity and stability.
Similar to phage display, the peptides in bacterial display are expressed on the plasma membrane as conjugated proteins. However, our system allows inducible expression, giving researchers control over the process.
Furthermore, we accept customer-supplied vectors, ensuring clients retain exclusive rights to their constructs. This flexibility makes our platform ideal for both academic and commercial research.
Our Approach
At Rx Biosciences, we also use a yeast display system to monitor antibody maturation. We express antibodies on the yeast surface and bind them to antigens under increasing stringency. Using Fluorescence-Assisted Cell Sorting (FACS), we then isolate the antigen-binding yeast population. Finally, we purify the selected antibodies using affinity chromatography.
As a result, our approach supports high-throughput selection, detailed analysis, and robust antibody development—all while ensuring accuracy and reliability at every step.
Service Highlights
- Maximized, uniform & extensive randomization of peptides ranging between 8 to 30 amino acids achieved by NNK, Split-mix-split synthesis or Tri-mer codon strategies.
- Codon optimization is performed based on host cells in use to maximize the presence of amino acid sequences at the surface of the phage encoded by the inserted DNA sequences
- Internal control tags, such as cMyc, and/or purification tags are incorporated as per project requirement.
- Purification of oligos on polyacrylamide (denaturing and native) gel to avoid truncated inserts > 10^9 to 10^10 diverse clones
Request a Quote
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.