RNA Isolation
Rx Biosciences offers total RNA and mRNA isolation services from a variety of samples such as blood, animal tissues, plants, cells and other biological specimens. The RNA, isolated by us, is free from genomic DNA (DNAse I treated) and other contaminants (polysaccharides and phenolic compounds). The quality of the RNA is checked by measuring absorbencies at 260nm and 280 nm wavelengths and by performing denaturing agarose gels. The RNA thus isolated is highly suitable for microarray analysis, northern blotting, QRTPCR, in vitro translation, RNase protection assay, full-length gene cloning, and library construction.
Service Highlights
- High quality total RNA and mRNA
- Free from genomic DNA and salts
- Quantitation by fluorometric assay for very low amount samples (pg or ng quantity)
- Additional services such as preparation of labeled probes for microarrays, first strand synthesis for QRTPCR, mRNA isolation are also offered
- First strand synthesis
- Dot blots, Norther Blots
- Cy-3 and Cy-5 labeling
- Synthesis of cRNA
DNA Isolation
We specialize in the generation of primary, immortalized, stable and knock-out cell lines. We have generated several cell lines for drug screening, diagnostics, protein expression and purification.
Service Highlights
- Small as well as large scale isolation (micrograms to gram quantities)
- Purification by cesium chloride gradient or ion-exchange chromatography
- High throughput DNA isolation in 96-well format
- Ready for sequencing or PC
- Endotoxin level provided (if requested)
- From infected E. coli or insect cell
- Purification over sucrose density gradient by ultra-centrifugation
- Extensively dialyzed in TE buffer
- Q.C. on low percentage agarose gel and restriction enzyme digestion
- Un-sheared high molecular weight (>200 mega bases)
- Isolation from variety of tissues-(mammalian tissues, plants, bacteria, yeast and other challenging tissues rich in endogenous nucleases and phenolic compounds)
- Highly suitable for PCR, Southern Blot analysis, RAP
- D analysis, polymorphism studies and library construction
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