RNA Isolation
Rx Biosciences offers professional RNA isolation services for researchers who require high-quality total RNA and mRNA from various biological samples. Our RNA isolation services cover blood, animal tissues, plants, cultured cells, and other specimen types. By prioritizing purity and integrity, we ensure your RNA is free from genomic DNA (DNase I treated) and contaminants such as polysaccharides and phenolic compounds.
Service Highlights
- High quality total RNA and mRNA
- Free from genomic DNA and salts
- Quantitation by fluorometric assay for very low amount samples (pg or ng quantity)
- Additional services such as preparation of labeled probes for microarrays, first strand synthesis for QRTPCR, mRNA isolation are also offered
- First strand synthesis
- Dot blots, Norther Blots
- Cy-3 and Cy-5 labeling
- Synthesis of cRNA
DNA Isolation
We specialize in the generation of primary, immortalized, stable and knock-out cell lines. We have generated several cell lines for drug screening, diagnostics, protein expression and purification.
Service Highlights
- Small as well as large scale isolation (micrograms to gram quantities)
- Purification by cesium chloride gradient or ion-exchange chromatography
- High throughput DNA isolation in 96-well format
- Ready for sequencing or PC
- Endotoxin level provided (if requested)
- From infected E. coli or insect cell
- Purification over sucrose density gradient by ultra-centrifugation
- Extensively dialyzed in TE buffer
- Q.C. on low percentage agarose gel and restriction enzyme digestion
- Un-sheared high molecular weight (>200 mega bases)
- Isolation from variety of tissues-(mammalian tissues, plants, bacteria, yeast and other challenging tissues rich in endogenous nucleases and phenolic compounds)
- Highly suitable for PCR, Southern Blot analysis, RAP
- D analysis, polymorphism studies and library construction
Request a Quote
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.