Protein Purification
Protein Purification Services
Rx Biosciences provides expert protein purification services using bacterial, yeast, insect, and mammalian expression systems. From gene design and codon optimization to synthesis, cloning, and expression, we offer complete solutions.
Our purification techniques include gravity flow columns, low-pressure systems, and FPLC using Cytiva GE AKTA. Purity is verified by SDS-PAGE and size-exclusion chromatography (SEC) profiling.
Available Protein Purification Platforms
Service Highlights
- A comprehensive range of services including gene designing, cloning to protein purification, and beyond.
- Affinity tag of choice- Fc, 6X histidine, GST, MBP, Strep (II), Protein A/G, Biotin, E-tag, Calmodulin-binding peptide.
- Method and purity of your choice (affinity, ion-exchange vs SEC).
- Fast turnaround time with approximately 2-3 weeks for bacterial and yeast-expressed protein, 3-6 weeks for mammalian-expressed protein, and 6-8 weeks for insect-expressed protein.
- Project customization and technical guidance throughout the project.
- One-stop after purification services such as Immuno-flowcytometry, Circular Dichroism and NMR.
- One-stop after purification services such as Immuno-flowcytometry, Circular Dichroism and NMR.
Services offered
- Gene synthesis, cloning, and protein purification.
- Monoclonal and polyclonal antibody purification using Pharmacia protein A/G columns.
- Removal of a tag or fusion protein from the recombinant protein.
- Characterization of the purified protein by western blots, ELISA, CD, SEC profile, and flow cytometry.
- Purification of 13C and 15N labeled UltraPure proteins for NMR studies.
- Conjugation of a moiety of interest-such as PEG, fluorophore, biotin, or streptavidin.
- Protein Stability assay by Differential Loss in Fluorescence (DLF) and Circular Dichroism.
- Salt exchange, desalting, concentration determination, lyophilization and vialing.
Request a Quote
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.