Shotgun Library
Rx Biosciences offers shotgun library construction service for variety of samples including genomic, viral, BAC, PAC, and cosmid insert DNAs. The library is made in pUC 18 sequencing vector utilizing ‘Double Adapter’ method, the technique used in BCM-HGSC for completing the Human Genome Project.
Service Highlights
- Insert size of customer’s choice (typically 1-3kb)
- 1- 8x genome coverage
- Q.C. by restriction enzyme digestion and sequencing
- Libraries are supplied as glycerol stock, on LB-amp plates or ligated DNA
- Plating, picking, and arraying of colonies in 96- or 384-well plates
- Services related to physical mapping such as contig assembly, BLAST analysis and searching of database etc offered
Request a Quote
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.