Shotgun

 Rx Biosciences offers expert shotgun library construction services for a wide range of DNA sources, including genomic, viral, BAC, PAC, and cosmid insert DNAs. This technique is essential for applications such as genome mapping, metagenomic studies, and next-generation sequencing projects.

Our shotgun library construction process uses the high-efficiency pUC18 sequencing vector combined with the “Double Adapter” method. This method, originally developed and used at the Baylor College of Medicine Human Genome Sequencing Center (BCM-HGSC), played a key role in the successful completion of the Human Genome Project. By following this proven strategy, we ensure high-quality libraries with minimal bias and maximum coverage.

Each library is carefully prepared under strict quality control protocols to maintain accuracy and consistency. Whether your research involves prokaryotic or eukaryotic genomes, our team customizes the library construction process to meet your project’s goals and sample type.

In addition, Rx Biosciences provides full support throughout the project—from sample preparation guidance to sequencing compatibility checks—making sure you get the most value and scientific insight from your data.

If you are looking for reliable, precise, and efficient shotgun library construction, our team is ready to assist with cutting-edge tools and extensive expertise.