Cosmid/ Fosmid Library

Rx Biosciences provides expert solutions for genomic library construction, offering fully customized services using both cosmid and Fosmid vectors. Our process supports a wide range of research applications by delivering highly reliable and consistent genomic libraries.

We begin by preparing genomic DNA through two methods: random shearing or restriction enzyme digestion. Depending on the project requirements, we select the most suitable technique to ensure optimal DNA integrity and size distribution. For digestion, we typically use Sau3A1, a proven enzyme for high-yield results.

Following DNA preparation, we isolate high molecular weight DNA and fractionate it over a sucrose density gradient. This critical step reduces shearing and preserves the quality of the DNA. We then select DNA fragments within the 30 to 40 kb range. After pooling and polishing these fragments, we ligate them to the appropriate cosmid or Fosmid vector.

Once the ligation is complete, we package the DNA and introduce it into a suitable host cell for propagation. This stage helps ensure the stability and accuracy of the genomic library.

To guarantee performance, each library undergoes rigorous quality control testing before delivery. Our clients receive fully validated libraries that are ready for downstream applications.

With over a decade of experience, Rx Biosciences is dedicated to delivering high-quality genomic library construction services that meet the unique needs of your research. From start to finish, our team ensures precision, consistency, and reliability in every project.