Subtracted cDNA Library
Rx Biosciences specializes in subtracted cDNA library preparation, a powerful technique used to selectively remove common gene messages found in both control and experimental samples. This process results in a library enriched with differentially expressed genes, which is crucial for gene expression studies.
Our subtracted cDNA library preparation uses an in-house subtraction method or PCR-based techniques to construct high-quality libraries. Throughout each stage of the library construction, we perform rigorous quality control checks to ensure accuracy and reproducibility. This careful process guarantees that the final library contains the most relevant gene sequences for your research.
The subtracted cDNA libraries prepared by Rx Biosciences are highly suitable for high-throughput sequencing projects. These libraries enable researchers to focus on genes that show significant expression differences, accelerating discovery and analysis in various biological studies.
Moreover, our experienced team tailors the subtraction and library preparation protocols to meet specific project needs. Whether working on small-scale experiments or large genomic studies, Rx Biosciences provides reliable and efficient subtracted cDNA library preparation services to support your research goals.
By choosing Rx Biosciences for your subtracted cDNA library preparation, you gain access to advanced technology, expert knowledge, and strict quality standards, ensuring your projects progress smoothly and effectively.
Our Approach
Need approach
Service Highlights
- Choose any vector
- Subtraction is confirmed at various steps by northern, Q-RT PCR, colony hybridization and/or high throughput sequencing
- Mirror Orientation Selection (MOS) to eliminate false positive clones from SSH library
- Virtual northern blotting
- Receive two size fractionated libraries (0.4kb-2.0kb and 2.0 kb and up) for each sample
- 90-100% recombination efficiency
- >5 million primary clones
- Phage resistant competent cells used as host for propagation
- 250-500 µg endo-toxin free transfection grade DNA supplied with the library
- Semi-solid amplification procedure adapted for amplification to prevent biased clone representation
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