Custom Circular RNA Synthesis

Rx Biosciences provides comprehensive custom circular RNA synthesis and analysis services to support advanced RNA research and applications. Our process consists of three key steps. First, we clone the CircRNA corresponding gene(s) into a plasmid vector. Next, we utilize T7 RNA polymerase for in vitro transcription and purify the mRNA. Finally, we enzymatically couple both ends of the linear mRNA to create the circular form. Any remaining linear RNA gets eliminated through digestion with RNase R. The resulting CircRNA is then purified using urea polyacrylamide gel, followed by washing with RNase-free water.

Our Approach

To ensure quality and reliability in custom circular RNA synthesis, the final CircRNA undergoes rigorous testing. These include absorbance measurements at 260 and 280 nm, gel electrophoresis, and HPLC analysis. Additionally, CD spectra are generated for both linear and circular mRNA for comparison, helping confirm the circularization.

Highlights of our service include the ability to create CircRNA with various backbones suitable for transfection and expression in eukaryotic cells. We also assess the efficiency and stability of the synthesized CircRNA through in vitro transcription in a specified cell line.

At Rx Biosciences, we aim to provide reliable and high-quality custom circular RNA synthesis tailored to meet specific research needs. Whether your goal is functional validation, therapeutic application, or molecular study, our expert team ensures each step is executed with precision and care.

Service Highlights
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