Normalized

Rx Biosciences constructs normalized cDNA libraries in which high-copy gene sequences are selectively removed, resulting in the enrichment of low-copy and rare genes within the library. This normalization process is crucial for improving the representation of rare transcripts that are often underrepresented in traditional cDNA libraries. As per customer request, we employ either of two advanced normalization technologies. In one method, double-stranded cDNA clones from a regular library are converted into single-stranded circles, which are then hybridized with complementary RNA (cRNA). The unhybridized single-stranded circular plasmids—representing low-copy genes—are then converted back into double-stranded plasmids for library construction. Alternatively, normalization can be performed at the mRNA level before constructing the library, selectively reducing high-copy transcripts.

Additionally, depending on the sample quality and customer requirements, a PCR-based normalization technique may be utilized. This method further ensures that the normalized cDNA libraries are enriched for rare and low-abundance transcripts, which are often critical for discovering novel genes or understanding complex gene expression profiles.

Our expert team customizes the normalization approach based on the specific needs of each project, ensuring that the final library provides comprehensive coverage of both common and rare gene sequences. These normalized cDNA libraries are ideal for applications including gene discovery, transcriptome analysis, and functional genomics studies, providing researchers with a more balanced and representative view of the expressed genome.