Library Construction by Random Shearing

We begin DNA library construction by isolating high molecular weight genomic DNA and embedding it in agarose beads. Next, we digest this DNA using restriction enzymes like HindIII, EcoRI, BamHI, Sau3A1, or ApoI. These enzymes cut the DNA into fragments suitable for library preparation.

To improve the diversity of fragments in the final library, we offer an enhanced method. This includes digesting the DNA separately with different enzymes, building individual libraries, and then pooling them. This strategy boosts the genomic representation in the final product.

For cloning, we use trusted vectors such as pCC1BAC, pIndigoBAC5, and pBleoBAC11. If customers prefer using their own vectors, we support that option too. These vectors ensure high-quality and stable insertion of genomic fragments.

Our method protects DNA integrity. Embedding DNA in agarose minimizes shearing and keeps the fragments intact. This step is critical for working with large genomes or long inserts.

We focus on precision in every stage of DNA library construction. Our goal is to deliver libraries that are reliable and diverse. These libraries are ideal for sequencing, gene cloning, and other genomic studies.

Each step in our process is optimized for quality. From digestion to vector selection and pooling, we maintain strict control to ensure excellent results. Our approach gives researchers a solid foundation for their work in genomics.