Our Approach
Rx Biosciences offers high-quality nylon membranes for hybridization. We robotically print these membranes using bacterial cells sourced from 96- or 384-well plates. As a result, researchers benefit from reliable and reproducible outcomes, especially when using hybridization-based screening methods.
To ensure exceptional performance, we use one of the best grades of nylon membranes available. This guarantees durability, high binding efficiency, and wide compatibility with various detection methods. Moreover, we pre-process our high-density membranes with great care, so they arrive ready-to-use. This eliminates extra preparation steps and ultimately saves valuable lab time.
Importantly, our nylon membranes for hybridization support experiments using both radioactive and non-radioactive DIG-labeled probes. Therefore, researchers can choose the detection method that best suits their safety standards, workflow, and budget.
Additionally, each membrane comes with a pre-printed template. This template corresponds directly to the original 96- or 384-well plates, making it easier to identify and retrieve positive clones. Consequently, users can minimize handling errors and locate desired clones with precision.
Whether you’re performing colony hybridization, screening for gene expression, or mapping genomic elements, our nylon membranes for hybridization offer a dependable and efficient solution. They’re designed to streamline your experiments and boost productivity in the lab.
At Rx Biosciences, we remain committed to delivering high-performance materials that support scientific discovery—both in academic institutions and industrial R&D environments.
Commonly used gridding patterns
- Membrane with duplicate clones:
- Membrane with single clones: A 22.5 cm x 22.5cm membrane printed with 9,216 or 18,432 clones per filter
- A 22.5 cm x 22.5cm membrane printed with 18432 or 36,864 clones per filter in duplicate.
Gridding Layout
A 22.5 cmx22.5 cm membrane is divided into 6 areas. Each area is spotted with clones at a low or higher density based as per requirement of the project. The area of the membrane, number of clones and gridding pattern is provided in the following table

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