Conventional Monoclonal Antibody Production
Rx Biosciences offer conventional monoclonal antibody production service based on the classic technique for production of monoclonal antibodies (Kohler and Milstein’s). With over 10 years of industry experience, we consult with our clients on the design and expression of the antigen. We have made numerous antibodies for customers ranging from academic laboratories and bio-pharmaceutical industry.
Our Approach
Rx Biosciences offers a range of options for developing monoclonal antibodies. Our core service includes immunization of the mice, hybridoma fusion, screening and cloning, adapting to cloning supplement free media and scale up/purification of lead candidates.
The steps for custom antibody generation workflow are shown below:
Stage I: Immunization of 5 mice (2 1/2 weeks)
Stage II: Fusion screening and selection of positive hybridomas (2 weeks)
Stage III: Cloning to produce a hybridoma cell line (2 weeks)
Stage IV: Adaption to cloning supplement free media (1 week)
Stage V: – Scale up/purification of lead candidates (1 week)
Each step is optional, as we offer a truly custom antibody service and strive to meet all of our customers’ specific needs. We are confident we can support your custom antibody development requirements throughout all stages of polyclonal antibody manufacturing.
Service Highlights
- We offer services for antigen preparation, including cloning, expression and purification of recombinant proteins.
- Q.C. by ELISA, western blotting and Biacore.
- 10 positive clones are delivered at the end of the project.
- Purification over protein A/G column.
- Quick turn around time of about 8-10 weeks.
Request a Quote
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.