Custom Phage Display Antibody Library
Rx Biosciences offers custom phage display library construction for antibody screening and development. We build high-quality scFv and Fab libraries from species including mouse, human, rabbit, chicken, and llama.
Each phage display library is developed using a phagemid vector, with either variable light (vL) or heavy (vH) chains cloned separately, or both together, connected by a linker. These libraries are ideal for discovering and producing monoclonal antibodies.
Whether for research or therapeutic use, our custom phage display libraries provide the flexibility and specificity needed for reliable antibody screening.
Service Highlights
- scFv Light Chain (vL) antibody library in a phagemid vector
- scFv Heavy Chain (vH) antibody library in a phagemid vector
- Construction of recombinant vL and vH chimeric antibodies in a phagemid vector
- Construction of Fab antibody library
- Construction of (Fab) 2 antibody library
- Construction of a library of nanobodies
- Construction of a bi-specific antibody library (with both domains, recognizing different targets)
- Construction of a bi-specific ligand-antibody conjugate (one domain acts like a antibody while other domain acts like a ligand)
- Construction of camelid and IgNAR antibody libraries
- Secreted expression in periplasm of E. coli.
- Soluble expression in cytoplasm of E. coli.
- Insoluble expressed as inclusion bodies
- Secreted expression in 293 and CHO cells.
- Affinity tag: 6xHis tag
- Molecular format: insertion of SUMO between His6 tag and protein of interest (POI)
- Purification: IMAC (Immobilized Metal Affinity Chromatography)
- Tag removal after purification: cleavage at the C terminus of SUMO with SUMO protease
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