Immunized Phage Display Antibody Library
Our expertise lies in creating custom phage display antibody libraries by combining immunological techniques with phage display technology. We immunize animals such as mice, rabbits, chickens, or llamas with the target antigen. After confirming a strong immune response via ELISA or western blotting, we isolate PBMCs or spleen cells to extract the necessary genetic material. We then amplify the cDNA for the vL and vH genes and clone it into a phagemid vector to construct a high-quality phage display antibody library.
The inserted cDNA is optimized for antibody expression and purification in bacterial or human cell lines. Our libraries contain clones expressing high-affinity antibodies on phage surfaces, which can be quickly screened. Moreover, antibody isolated from these libraries can be easily modified — humanized or chimerized — for therapeutic or research applications. Once suitable clones are identified, they can be scaled up for large-scale production.
Our Approach
We utilize animals such as mice and rabbits, which generate strong immune responses to most antigens. Rabbits are particularly effective against small haptens and serve as an excellent alternative to mice when producing monoclonal antibodies. Additionally, llamas and sharks produce smaller antibody fragments, known as nanobodies, which offer unique advantages. For sharks, we use the IgNAR V scaffold sequence to develop the antibody library.
After immunization, we collect blood or spleen from the best responders and amplify immunoglobulin-specific cDNA to construct the library. We also provide bleeds so you can perform your own testing and validation.
We prepare Custom immunized phage display antibody libraries for the following animals:
- Mice
- Rat
- Rabbit
- Chicken
- Llama
- Shark IgNAR V
Service Highlights
- Different antibody format and display type
- scFv and Fab libraries with specific format and tags
- 1 x 109 in-frame antibody clones
- Quick turn around time
- Competitive pricing
Request a Quote
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.