Subtracted cDNA Library
Rx Biosciences prepares subtracted cDNA libraries after selectively removing the messages that are present in both, the control as well as the experimental samples. The subtracted library prepared by us is enriched in differentially expressed genes. Using in-house subtraction procedure or other PCR based techniques the libraries are constructed involving rigorous Q.C. at all the stages of the library construction. The libraries prepared by us are highly suitable for high throughput sequencing projects.
Our Approach
Need approach
Service Highlights
- Choose any vector
- Subtraction is confirmed at various steps by northern, Q-RT PCR, colony hybridization and/or high throughput sequencing
- Mirror Orientation Selection (MOS) to eliminate false positive clones from SSH library
- Virtual northern blotting
- Receive two size fractionated libraries (0.4kb-2.0kb and 2.0 kb and up) for each sample
- 90-100% recombination efficiency
- >5 million primary clones
- Phage resistant competent cells used as host for propagation
- 250-500 µg endo-toxin free transfection grade DNA supplied with the library
- Semi-solid amplification procedure adapted for amplification to prevent biased clone representation
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