Custom Circular RNA Synthesis
Rx Biosciences provides comprehensive circular RNA synthesis and analysis services. Our process consists of three key steps. First, we clone the CircRNA corresponding gene(s) into a plasmid vector. Next, we utilize T7 RNA polymerase for in vitro transcription and purifying the mRNA. Finally, we enzymatically couple both ends of the linear mRNA to create the circular form. Any remaining linear RNA gets eliminated through digestion with RNase R. The resulting CircRNA is then purified using urea polyacrylamide gel followed by washing with RNase-free water.
Our Approach
To ensure quality, the final CircRNA undergoes rigorous testing including absorbance measurements at 260 and 280 nm, gel electrophoresis, and HPLC analysis. Additionally, CD spectra are generated for both linear and circular mRNA for comparison.
Highlights of our service include the ability to create CircRNA with various backbones for transfection and expression in eukaryotic cells. We also assess the efficiency and stability of the CircRNA through in vitro transcription in a specified cell line.
Service Highlights
- Select the vector of your choice such as two-hybrid, retroviral or any other toxic vector
- Receive two size fractionated libraries (0.4kb-2.0kb and 2.0 kb and up) for each sample
- 90-100% recombination efficiency
- >5 million primary clones supplied
- Phage resistant host competent cells used for propagation
- 500 µg endo-toxin free DNA supplied with the library
- Semi-solid amplification procedure adapted for amplification preventing unbiased clone representation
Request a Quote
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.