Regular cDNA & EST
Rx Biosciences prepares regular cDNA and EST libraries from various sources ranging from bacteria, plants, sea animals, algae, fungi, cacti, nematodes, pathological samples and others. We are specialized in constructing the libraries from micro-quantities of total RNA usually obtained from hard to procure blood samples, tissues or cells. The libraries are highly suitable for the generation of EST data base, screening and other purposes.
Our Approach
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Service Highlights
- Select the vector of your choice such as two-hybrid, retroviral or any other toxic vector
- Receive two size fractionated libraries (0.4kb-2.0kb and 2.0 kb and up) for each sample
- 90-100% recombination efficiency
- >5 million primary clones supplied
- Phage resistant host competent cells used for propagation
- 500 µg endo-toxin free DNA supplied with the library
- Semi-solid amplification procedure adapted for amplification preventing unbiased clone representation
Request a Quote
Ready to start your project? Send us a message and we’ll provide you with a customized quotation for your project’s requirements.