Phage Display cDNA Library
cDNA libraries are constructed in the following systems:
T7 Select series of vectors (Novagen).
M13 based phagemid vectors
Yeast display (YD) vectors
Ribosome display systems
Our Approach
Rx Biosciences uses yeast display system for the monitoring of the antibody maturation. An antibody expressed at the surface is allowed to bind to the antigen at increasing stringency and antigen-binding yeast cell population is sorted out using Fluorescence Assisted Cell Sorting (FACS). The antibody is then purified over an affinity column.
Service Highlights
- No need for large amount of RNA. Only few hundred nanogram of total RNA or mRNA is enough for library construction!
- Directional library generated
- No digestion of cDNA as we do not use EcoRI/HindIII during cloning
- Larger cDNA expressed on the phage surface
- Higher clone diversity
- Minimum 10 million primary clones delivered
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