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Custom Phage Display Antibody Library

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Empower your genome research by using our next generation sequencing
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Cell-free and cell-based assays for drugs, antigens, proteins, enzymes and contaminants in biologics
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Unrivaled state-of-the-art genomic services through years of R&D
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Explore the power of Phage Display for drug discovery
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We are constantly looking to begin new relationships to nurture new ideas

Custom Phage Display Antibody Library

Rx Biosciences offers construction of mouse, human, rabbit, chicken and llamas scFv and Fab libraries in a phagemid vector. Either vL or vH are cloned separately or vL and vH are cloned together, separated by a linker region.  The libraries are good for screening and production of monoclonal antibodies.

+ scFv Light Chain (vL) antibody library in a phagemid vector
+ scFv Heavy Chain (vH) antibody library in a phagemid vector
+ Construction of recombinant vL and vH chimeric antibodies in a phagemid vector
+ Construction of Fab antibody library
+ Construction of (Fab) 2 antibody library
+ Construction of a  library of nanobodies
+ Construction of a bi-specific antibody library (with both domains, recognizing  different targets)
+ Construction of a bi-specific ligand-antibody conjugate (one domain acts like a antibody while other domain acts like a ligand)
+ Construction of camelid and IgNAR antibody libraries

Expression strategies:
  • Secreted expression in periplasm of E. coli.
  • Soluble expression in cytoplasm of E. coli.
  • Insoluble expressed as inclusion bodies
  • Secreted expression in 293 and CHO cells.
Purification strategies:
  • Affinity tag: 6xHis tag.
  • Molecular format: insertion of SUMO between His6 tag and protein of interest (POI).
  • Purification: IMAC (Immobilized Metal Affinity Chromatography).
  • Tag removal after purification: cleavage at the C terminus of SUMO with SUMO protease.

To receive a custom quote, please contact us. We look forward to hearing from you!

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